Cytotoxicity evaluation of methacrylate-based resins for clinical endodontics in vitro
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Date
2012Author
Wadajkar, Aniket S
Garza, Esteban G.
Ahn, Chul
Zhu, Qiang
Opperman, Lynn A.
Bellinger, Larry L.
Komabayashi, Takashi
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Show full item recordAbstract
This study examines the cytotoxicity of
Super-Bond C&B (SB-C&B), Super-Bond RC Sealer
(SB-RC), MetaSEAL (Meta), and AH Plus Sealer
(AH+). Freshly mixed and set materials (100 mg) were
prepared in vitro and placed in cell culture medium (1
mL) for the working time and for 6 h, respectively.
L929 cells seeded into 96-well plates at 5,000 cells/
well were incubated with the eluted medium (200 μL)
for 24 h. Cells cultured with medium alone served as
the control. Cytotoxicity was evaluated by MTS assay
and analyzed with ANOVA. In the freshly mixed
group, the average ± SD (%) for cell viability were
66.0 ± 13.6, 55.5 ± 15.6, 10.6 ± 0.7, and 8.9 ± 2.2 for
SB-C&B, SB-RC, Meta, and AH+, respectively. In
the set group, the average ± SD (%) for cell viability
were 100 ± 21.9, 81.8 ± 38.5, 24.9 ± 7.9, and 23.6 ± 10.0
for SB-C&B, SB-RC, Meta, and AH+, respectively.
SB-C&B and SB-RC are less cytotoxic than are Meta
and AH+.