Interplay between up-regulation of cytochrome-c-oxidase and hemoglobin oxygenation induced by near-infrared laser
Date
2016-08-03Author
Wang, Xinlong
Tian, Fenghua
Soni, Sagar S.
Gonzalez-Lima, F.
Liu, Hanli
Metadata
Show full item recordAbstract
Photobiomodulation, also known as low-level laser/light therapy (LLLT), refers to the use of red-tonear-infrared light to stimulate cellular functions for physiological or clinical benefits. The mechanism
of LLLT is assumed to rely on photon absorption by cytochrome c oxidase (CCO), the terminal enzyme
in the mitochondrial respiratory chain that catalyzes the reduction of oxygen for energy metabolism. In
this study, we used broadband near-infrared spectroscopy (NIRS) to measure the LLLT-induced changes
in CCO and hemoglobin concentrations in human forearms in vivo. Eleven healthy participants were
administered with 1064-nm laser and placebo treatments on their right forearms. The spectroscopic
data were analyzed and fitted with wavelength-dependent, modified Beer-Lambert Law. We found
that LLLT induced significant increases of CCO concentration (Δ[CCO]) and oxygenated hemoglobin
concentration (Δ[HbO]) on the treated site as the laser energy dose accumulated over time. A strong
linear interplay between Δ[CCO] and Δ[HbO] was observed for the first time during LLLT, indicating a
hemodynamic response of oxygen supply and blood volume closely coupled to the up-regulation of CCO
induced by photobiomodulation. These results demonstrate the tremendous potential of broadband
NIRS as a non-invasive, in vivo means to study mechanisms of photobiomodulation and perform
treatment evaluations of LLLT
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