Use Of Temperature Sensitive Mutants To Map Reovirus Induced Oncolysis
Tam, Ka Ian
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Reovirus is known to establish persistent infections in normal cells while selectively destroying transformed cells. Individual viruses from each of the ten groups of Reovirus is known to establish persistent infections in normal cells while selectively destroying transformed cells. Individual viruses from each of the ten groups of Reovirus temperature sensitive mutants were used to examine the gene or genes involved in the differential lysis activity. Temperature sensitive mutants were generated by chemical mutagenesis and have one of the ten genes impaired when growing at the non-permissive temperature, while they behave like wild type when growing at the permissive temperature. Normal cells and their transformed cells counterpart were used in this study: L929, WI-38, WI-38 VA13 2RA, T1, N1, and MYC-3. Reovirus maintains a persistent infection in normal cell lines: WI-38 cells and MMEC, but lyses the transformed cell lines WI-38 VA13 2RA and N1 cells. However, the tsE did not lyse the T1 and Myc3 cells while tsG did not lyse the Myc3 cells. The tsE and tsG have their gene lesion in S3 and S4 genes respectively, which demonstrated that σNS and σ3 are involved in the differential lysis activities.