Browsing Department of Chemistry and Biochemistry by Title
Now showing items 91-110 of 247
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FLUORINATED ALCOHOL INDUCED SUPRAMOLECULAR BIPBASIC SYSTEMS IN PROTEOMICS AND LIPIDOMICS
(2021-05-28)The Fluoroalcohol induced Supramolecular Biphasic (FAiS-BP) systems are composed of a supramolecular phase which is a highly concentrated mixture of a fluoroalcohol and an amphiphile, and a separate aqueous-rich phase. The ... -
FLUOROALCOHOL INDUCED SUPRAMOLECULAR BIPHASIC SYSTEMS OF ZWITTERIONIC SURFACTANTS, MIXED AMPHIPHILES, AND POLAR ORGANIC SOLVENTS IN PROTEOMICS AND ENVIRONMENTAL ANALYSIS
(2021-12-17)In this research, we have investigated several HFIP-induced biphasic systems of zwitterionic surfactants like CHAPS and DMMAPS and their mixed amphiphilic systems for the extraction, enrichment, and fractionation of mixture ... -
Fundamental And Applied Studies Of An Ambient Ionization Technique: Continuous Flow-extractive Desorption Electrospray Ionization - Mass Spectrometry
(Chemistry & Biochemistry, 2014-07-14)Continuous flow extractive desorption electrospray ionization (CF-EDESI) is a novel ambient ionization technique that was developed to help analyze samples from non-ESI-friendly solvents, manipulate protein charge states, ... -
FUNDAMENTAL STUDIES ON ULTRAFAST CHIRAL AND ACHIRAL SEPARATIONS IN LIQUID CHROMATOGRAPHY AND SUB/SUPERCRITICAL FLUID CHROMATOGRAPHY
(2017-05-03)This thesis is based on fundamental contributions in the field of ultrafast chiral and achiral separations using liquid and sub/supercritical fluid chromatography. The high efficiency sub-2 μm particles of narrow particle ... -
FUNDAMENTAL STUDIES ON ULTRAFAST SEPARATIONS AND DEVELOPMENT OF GEOPOLYMER BASED BASE-STABLE STATIONARY PHASE MEDIA
(2018-12-13)This thesis consists of two sections. In the first section fundamentals of ultrafast separations is discussed. Ultrafast chromatography is an emerging area of separation science due to its applicability in high-throughput ... -
F₄₂₀ cofactor dependent enzymes : kinetic, mechanistic and structural characterization of F₄₂₀-dependent glucose-6-phosphate dehydrogenase (FGD) from Mycobacterium tuberculosis
(2017-08-02)F₄₂₀-dependent glucose-6-phosphate dehydrogenase (FGD) from Mycobacterium tuberculosis (Mtb) is one of a few enzymes that utilize the unique F₄₂₀ cofactor for catalysis. FGD catalyzes the first committed step of the ... -
Gas Chromatography And Metal Analysis Of Produced Water Treated With A Household Commercial Filter
(2017-05-15)Unconventional oil and gas extraction activities, including hydraulic fracturing, are a collection of processes used to liberate significant quantities of natural gas and oil in the United States. Many extraction sites are ... -
Genetic Engineering Of Proteins For Biotechnology Applications
Heme b (protoporphyrin IX or protoheme) plays a major role in the structure and function in proteins such as myoglobin (Mb), hemoglobin (Hb), horseradish peroxidase (HRP), catalase, cytochrome P450 as well as catalase ... -
Genetically Modified Myoglobin As A Mimic For Heme Enzymes
(Chemistry & Biochemistry, 2013-07-22)This study addresses the mechanistic relationships between formation of reactive oxygen species (ROS) and their catalytic oxidation functions in oxygenation and peroxygenation reactions in heme enzymes. Even though both ... -
Headspace-Solid Phase Micro Extraction – Gas Chromatography – Mass Spectrometry (HS-SPME-GC-MS) Analysis for Oak Barrel Flavor Compounds in Whiskey
(2021-12-13)This study presents a method to simultaneously detect both fault compounds and oak barrel flavor compounds in whiskey using headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). A major ... -
HIGH CONFIDENCE IDENTIFICATION OF CROSS-LINKED PEPTIDES BY MASS SPECTROMETRY CLEAVABLE CROSS-LINKING TECHNOLOGIES
(2019-12-06)Mass spectrometry (MS)-based chemical cross-linking technique is a potential tool for understanding the protein structure and protein-protein interactions. However, low abundances of cross-linked products and the data ... -
HIGHLY FLUORINATED, COINAGE METAL COMPLEXES OF TRIS(PYRAZOLYL)BORATES WITH ADDITIONAL ELECTRON WITHDRAWING GROUPS AND PYRAZOLATE π-STACKINGS WITH ARENES
(2016-05-11)Tris(pyrazolyl)borates are very popular ligands in inorganic chemistry, bioinorganic chemistry, and organometallic chemistry due to their attractive properties. It is possible to extensively modify the steric and electronic ... -
Identification of acidic residues in proteins by selective chemical labeling and tandem mass spectrometry
(2023-08-15)**Please note that the full text is embargoed until 08/01/2025** Proteins play important role in carrying out a wide range of cellular activities. They are subjected to various post-translational modifications (PTMs) by ... -
IDENTIFICATION OF PROTEOLYTIC CLEAVAGE AND ARGININE MODIFICATIONS BY CHEMICAL LABELING AND MASS SPECTROMETRY
(2017-12-06)Post-translational modification of proteins plays a significant role in the regulation of cellular processes. During the translation, process proteins undergo different modifications, which are known as post-translational ... -
IMPROVED FISCHER-TROPSCH SYNTHESIS BY THERMAL MANAGEMENT OF THE CATALYST
(2021-08-10)The research in this dissertation consists of a novel catalyst design which is aimed at helping manage the temperature instabilities and heat-transfer within a catalyst particle and maintaining an isothermal temperature ... -
Improved sample preparation method for LC-MS/MS based cellular proteomics and co-immunoprecipitation cross-linking proteomics study of Toll-like receptor 4
(2019-08-02)Mass spectrometry-based methods are now widely practiced in large-scale proteomics studies. Unfortunately, sample loss in proteomics hinders complex analysis in proteomics, and immune precipitation (IP) mass spectrometry-based ... -
INCREASED HYDRIDE TRANSFER RATES WITHIN T09A MUTANT OF F420:H2 NADP+ OXIDOREDUCTASE
(2016-05-18)F420:H2 NADP+ oxidoreductase (Fno) is an F420 dependent enzyme found in methanogens and sulfate reducing archaea. Fno catalyzes the reversible reduction of NADP+ to NADPH through the transfer of a hydride involving F420 ... -
Increasing Lipophilicity Of Redox Active Ruthenium Complexes As A Means To Enhance Cytotoxicity And Reduce Animal Toxicity
(Chemistry & Biochemistry, 2013-03-20)The dinuclear and monomeric ruthenium(II) polypyridyl complexes [(phen)2Ru(tatpp)Ru(phen)2]Cl4 (P) and monomer [(phen)2Ru(tatpp)]Cl2 (MP) are promising candidates for anticancer drug development in terms of the observed ... -
INTACT PROTEIN DETECTION, SEPARATION, AND QUANTITATION USING LIQUID CHROMATOGRAPHY - TRIPLE QUADRUPOLE MASS SPECTROMETRY
(2016-08-10)There is an increasing demand for protein detection and quantitation in biological fluids for disease detection, protein therapeutics monitoring, and drug development response control. Current methods use highly sensitive ... -
INTRACELLULAR BIOCHEMICAL TARGETS OF RUTHENIUM POLYPYRIDYL COMPLEXES IN MULTIPLE CANCER CELL MODELS
(2016-12-16)The ruthenium (II) polypyridyl complexes [(phen)2Ru(tatpp)Ru(phen)2]4+, RPC4 and [(phen)2Ru(tatpp)]2+, RPC3 are promising anticancer candidates due to their observed cytotoxic effect against multiple cancer cell lines. ...