Evolution of allostery and conformational dynamics of caspases involved in the extrinsic pathway of apoptosis
Date
2023-04-25Author
Karadi Giridhar, Mithun Nag
0000-0003-0520-5921
Metadata
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Caspases are a class of cysteine proteases that play a crucial role in programmed cell death (apoptosis). We complete an evolutionary picture of folding of caspases in the extrinsic pathway of apoptosis. The folding models show that the conformational free energy of the monomeric fold is conserved in the extrinsic pathway of apoptosis. In addition, with molecular dynamics and mass spectrometry we also show that the small subunit is less stable than the large subunit and charges destabilize allosteric regions in caspases allowing for dynamics. In order to gain insight into the evolution of allostery in caspases across different species, we employ an evolutionary strategy that combines experimental and computational methods. The findings provide mechanistic details of allostery in caspases, highlighting a highly conserved process that has evolved diverse mechanisms of packing hydrophobic residues in the core, which controls the architecture of the active site to fine-tune the specificity and activity of caspases. Further we identify residues and interactions that mediate allosteric regulation and substrate recognition, which has implications for the development of novel drugs to treat a wide range of diseases, including cancer and autoimmune disorders. Lastly, we show that amino acid evolution in the small subunit drives the evolution of varied oligomerization properties in initiator and effector subfamilies of the extrinsic pathway of apoptosis.