Enantiomeric Chromatographic Separations And Ionic Liquids In Gas Chromatography

Abstract

Due to the different therapeutic values of optical isomers, the production of enantiomerically pure drugs is of growing demand in pharmaceutical, biotechnology, and agrochemical industries. Today, gas chromatography (GC) and high performance liquid chromatography (HPLC) are the most prevalent analytical techniques for the enantiomeric separation of chiral molecules. Although the two techniques can have very different separation mechanisms, they are highly complementary to each other. Together, they can accomplish enantiomeric separations for large numbers of compounds of different functionalities. A great variety of chiral stationary phases (CSPs) have been developed over the years. However, since they are the heart of these enantiomeric separation processes, the development of new CSPs will continue to be of considerable academic and industrial interest.In this dissertation, I present method development strategies, for enantiomeric and stereoisomeric separations using GC and HPLC. Also the development, characterization and application of novel ionic liquid-based GC stationary phases, and the development and evaluation of IL-based charge cyclodextrin GC-CSPs and dalbavancin-based HPLC-CSPs are discussed.