Browsing Department of Chemistry and Biochemistry by Author "Pierce, Brad"
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Applications Of Circular Dichroism (CD) Spectroscopy As An Educational Tool For Undergraduate Students And Monitoring Protein Conformational Changes Induced By Ligand Binding
Van Der Kar, Catherine Ann (Chemistry & Biochemistry, 2013-03-20)The work reported here provides an overview on the applications of circular dichroism (CD) spectroscopy for the study of protein secondary structure. This project has two specific goals. First, to develop and validate an ... -
Biochemical, Kinetic And Spectroscopic Characterizations Of Non-heme Iron Oxygenase Enzymes
Subedi, Bishnu P.MiaE is a non-heme diiron enzyme which catalyzes the O2-dependent hydroxylation of selected tRNA-nucleotides as part of a multienzyme posttranscriptional hypermodification pathway. This tRNA-modifying enzyme is postulated ... -
Mechanistic Study Of Cysteine Dioxygenase, A Non-heme Mononuclear Iron Enzyme
Li, Wei (Chemistry & Biochemistry, 2014-09-17)Cysteine dioxygenase (CDO) is an non-heme mononuclear iron enzymes that catalyzes the O2-dependent oxidation of L-cysteine (Cys) to produce cysteine sulfinic acid (CSA). CDO controls cysteine levels in cells and is a ... -
A Non-heme Diiron Monooxygenase From Salmonella Typhimurium: Biochemical Characterization Of TRNA-modifying MiaE
Zhang, Siai (Chemistry & Biochemistry, 2013-07-22)MiaE is a carboxylate bridged, non-heme diiron monooxygenase which catalyzes the O2-dependent hydroxylation of a hypermodified tRNA nucleoside at position 37 (2-methylthio-N-6-isopentenyl adenosine (37)-tRNA) [designated ... -
Spectroscopic and mechanistic investigation of the enzyme mercaptopropionic acid dioxygenase (MDO), the role of the conserved outer-sphere 'catalytic triad' in directing promiscuous substrate-specificity
Sardar, Sinjinee (2018-05-16)Mercaptopropionate dioxygenase (MDO) from Aztobacter Vinelandii is a non-heme mononuclear iron enzyme that catalyzes the oxygen dependent conversion of 3-mercaptopropionic acid (3mpa) to produce 3-sulfinopropinioic acid ... -
Thiol Dioxygenases: An Investigation Into The Mechanisms Of Cysteine Dioxygenase And 3-mercaptopropionic Acid Dioxygenase
Crowell, Joshua (Chemistry & Biochemistry, 2015)Thiol dioxygenase (TDO) enzymes catalyze the molecular oxygen-dependent oxidation of sulfur containing amino acid derivatives. The loss of the ability to regulate thiols has been linked to a number of neurological diseases. ...