Browsing PhD Dissertations - DO NOT EDIT by Title
Now showing items 68-87 of 173
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Genetic Engineering Of Proteins For Biotechnology Applications
Heme b (protoporphyrin IX or protoheme) plays a major role in the structure and function in proteins such as myoglobin (Mb), hemoglobin (Hb), horseradish peroxidase (HRP), catalase, cytochrome P450 as well as catalase ... -
Genetically Modified Myoglobin As A Mimic For Heme Enzymes
(Chemistry & Biochemistry, 2013-07-22)This study addresses the mechanistic relationships between formation of reactive oxygen species (ROS) and their catalytic oxidation functions in oxygenation and peroxygenation reactions in heme enzymes. Even though both ... -
HIGH CONFIDENCE IDENTIFICATION OF CROSS-LINKED PEPTIDES BY MASS SPECTROMETRY CLEAVABLE CROSS-LINKING TECHNOLOGIES
(2019-12-06)Mass spectrometry (MS)-based chemical cross-linking technique is a potential tool for understanding the protein structure and protein-protein interactions. However, low abundances of cross-linked products and the data ... -
HIGHLY FLUORINATED, COINAGE METAL COMPLEXES OF TRIS(PYRAZOLYL)BORATES WITH ADDITIONAL ELECTRON WITHDRAWING GROUPS AND PYRAZOLATE π-STACKINGS WITH ARENES
(2016-05-11)Tris(pyrazolyl)borates are very popular ligands in inorganic chemistry, bioinorganic chemistry, and organometallic chemistry due to their attractive properties. It is possible to extensively modify the steric and electronic ... -
IDENTIFICATION OF PROTEOLYTIC CLEAVAGE AND ARGININE MODIFICATIONS BY CHEMICAL LABELING AND MASS SPECTROMETRY
(2017-12-06)Post-translational modification of proteins plays a significant role in the regulation of cellular processes. During the translation, process proteins undergo different modifications, which are known as post-translational ... -
IMPROVED FISCHER-TROPSCH SYNTHESIS BY THERMAL MANAGEMENT OF THE CATALYST
(2021-08-10)The research in this dissertation consists of a novel catalyst design which is aimed at helping manage the temperature instabilities and heat-transfer within a catalyst particle and maintaining an isothermal temperature ... -
Improved sample preparation method for LC-MS/MS based cellular proteomics and co-immunoprecipitation cross-linking proteomics study of Toll-like receptor 4
(2019-08-02)Mass spectrometry-based methods are now widely practiced in large-scale proteomics studies. Unfortunately, sample loss in proteomics hinders complex analysis in proteomics, and immune precipitation (IP) mass spectrometry-based ... -
INTACT PROTEIN DETECTION, SEPARATION, AND QUANTITATION USING LIQUID CHROMATOGRAPHY - TRIPLE QUADRUPOLE MASS SPECTROMETRY
(2016-08-10)There is an increasing demand for protein detection and quantitation in biological fluids for disease detection, protein therapeutics monitoring, and drug development response control. Current methods use highly sensitive ... -
INTRACELLULAR BIOCHEMICAL TARGETS OF RUTHENIUM POLYPYRIDYL COMPLEXES IN MULTIPLE CANCER CELL MODELS
(2016-12-16)The ruthenium (II) polypyridyl complexes [(phen)2Ru(tatpp)Ru(phen)2]4+, RPC4 and [(phen)2Ru(tatpp)]2+, RPC3 are promising anticancer candidates due to their observed cytotoxic effect against multiple cancer cell lines. ... -
Investigation Of Redox-active Ruthenium(II) Polypyridyl Complexes As Potential Anti-cancer Drugs
(Chemistry & Biochemistry, 2009-09-16)Stereospecific binding of ruthenium metallointercalators with DNA is of particular interest because of the potential for improved targeting of specific sites in DNA. The present studies are focused on new metallointercalator, ... -
Investigation Of Ruthenium (II) Polypyridyl Dimers As Potential Chemotherapeutic Agents
(Chemistry & Biochemistry, 2007-08-23)The exploration of transition metal complexes as chemotherapeutic agents is still a relatively unexplored but promising area of research. Chapter 1 highlights the most successful anticancer drugs and potential drugs based ... -
Ion Exchanger Based Ion Generation And Removal Devices. Behavior And Applications
(Chemistry & Biochemistry, 2012-04-11)Ion exchangers have many important applications. In Ion chromatography (IC), ion exchangers are used as stationary phase and used in some of the key components. The innovations that make the modern IC the most popular ... -
Ionic Liquids In Analytical Chemistry And Calix[4]arene Chemistry Of NOx Gases
(Chemistry & Biochemistry, 2011-03-03)Application of ionic liquids (ILs) in analytical chemistry can be numerous. The use of ILs in analytical techniques including gas chromatography (GC), electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis ... -
Leveraging propargylic frameworks in the total synthesis of natural products containing a 2-aminoimidazole
(2019-08-30)Our group is mainly interested in the total synthesis of marine sponge-derived 2-aminoimidazole-containing alkaloids. A new family of 2-aminoimidazole alkaloids, the Leucetta alkaloids, is a group of >60, 2-aminoimidazole ... -
LIQUID FLOW MEASUREMENT: FROM INLINE HYDROCEPHALUS SHUNT FLOW MONITOR TO FLOWMETRY IN THE NANOLITER/MINUTE SCALE
(2019-08-13)This dissertation primarily focuses on designing and developing novel sensing strategies and instruments for ultra-low liquid flow measurement. An inline thermal flow sensor based on a single common thermistor has been ... -
Long non-coding RNAs in inflammation, macrophage activation, and inflammatory human diseases
(2023-08-28)**Please note that the full text is embargoed until 08/01/2025** Inflammation and immune signaling is central to infection and diseases. Growing evidence suggests that along with protein coding genes, long non-coding RNAs ... -
Mass Spectrometry-based Biomolecular Recognition And Response Factor Investigations Using Electrospray Ionization
(Chemistry & Biochemistry, 2008-09-17)Integrins are transmembrane proteins found in almost all cells. They play vital roles in many biological processes, such as angiogenesis and signal transduction, through their interactions with different extracellular ... -
MECHANISM AND USE OF PAIRED ION ELECTROSPRAY IONIZATION (PIESI) IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (HPLC-MS) FOR SENSITIVE ANALYSIS OF ANIONIC COMPOUNDS
(2016-04-18)In recent years, the paired ion electrospray ionization (PIESI) technique has been emerged as a promising alternative to overcome the inherent low sensitivity of negative ESI-MS. The current research focused on the further ... -
Mechanistic And Kinetic Studies On F420H2: NADP+ Oxidoreductase From Archeoglobus fulgidus
F420H2: NADP+ Oxidoreductase (Fno) is an F420 cofactor dependent enzyme that catalyzes the reversible reduction of NADP+ to NADPH by transferring a hydride from the reduced F420 cofactor. F420 cofactor is an essential ... -
MECHANISTIC STUDIES OF F420-DEPENDENT ENZYMES BY KINETIC ANALYSIS
(2021-08-10)The focus of this research is to elucidate the catalytic mechanism of F420-dependent enzymes using F420-dependent glucose-6-phosphate dehydrogenase (FGD) from Mycobacteria tuberculosis (Mtb) and F420H2:NADP+ Oxidoreductase ...